CELLvo™ Matrix Plus

CELLvo™ Matrix Plus is the next generation of CELLvo™ Matrix technology. CELLvo™ matrices are the only substrates that aim to recapitulate biochemical as well as structural and mechanical cues of the native niche by leaving the extracellular matrix intact. Cell-derived matrices create a biologically-relevant culture environment for a variety of cell types by providing all the cues cells expect to receive in vivo in our culture dishes. This means that instead of responding to foreign culture conditions, cells respond to our customers’ experimental variables. Initially developed for pluripotent stem cell culture, we have subsequently discovered that CELLvo™ Matrix Plus is the ideal substrate for cardiomyocyte maturation and culture. Try CELLvo™ Matrix Plus Today!

CELLVO™ MATRIX PLUS SUPPORTS iPSC EXPANSION

iPSC expansion can be a hassle. Pre-coated plates often have a short shelf-life, and coating wells with other products can be a challenge, especially when working with small-well formats. CELLvo™ Matrix Plus is already on the bottom of the dish and only needs to be rehydrated prior to seeding your cells by adding PBS. This eliminates potential variability from coating by hand and saves you time. In our experiments, CELLvo™ Matrix Plus supports comparable self-renewal of iPSCs relative to Matrigel, but with greater ease of use.

CARDIOMYOCYTES SPONTANEOUSLY ALIGN AND SHOW STRUCTURAL MATURATION

Cardiomyocyte maturation is a major obstacle to the use of iPSC-derived cardiomyocytes for basic research as well as pre-clinical drug and toxicity screening. Commercially-available iPSC-derived cardiomyocytes seeded onto CELLvo™ Matrix Plus rapidly and spontaneously align and become structurally mature. Many groups are using electrical pacing, 3-D microtissues with mechanical stimulation, or very long culture periods to achieve some degree of maturation. CELLvo™ Matrix Plus is an easy-to-use solution for cardiomyocyte maturation in 2D culture.

iPSC-derived cardiomyocytes spontaneously align and structurally mature when seeded on CELLvo™ Matrix Plus. Here, iCell Cardiomyocytes (CDI) are shown 7 days after seeding on CELLvo™ Matrix Plus.

iPSC-derived cardiomyocytes become binucleated during culture on CELLvo™ Matrix Plus.

iPSC-CARDIOMYOCYTES BECOME ROD-SHAPED & BI-NUCLEATED ON CELLVO™ MATRIX PLUS

Within 7 days of seeding on CELLvo™ Matrix Plus, a large percentage of commercially-available iPSC-derived cardiomyocytes become rod-shaped and bi-nucleated. These are markers of maturation seldom seen using traditional methods.

CELLVO™ MATRIX PLUS IMPROVES SELF-RENEWAL AND DIFFERENTIATION POTENTIAL OF MSCs

Mesenchymal stem cells cultured on CELLvo™ Matrix Plus have improved self-renewal and differentiation potential relative to TCP. The figure on the right demonstrates this by CFU-F (colony formation), CFU-AD (adipogenic differentiation), and CFU-OB (osteogenic differentiation). Additionally, MSC proliferation is dramatically increased on CELLvo™ Matrix Plus, relative to other substrates.

Pre-expansion on CELLvo™ Matrix Plus improves self-renewal and differentiation of MSCs relative to tissue-culture plastic (TCP).

Cardiomyocytes on CELLvo™ Matrix Plus become a structurally mature monolayer after 7 days in culture.

CARDIOMYOCYTES FORM A UNIFORM, STRUCTURALLY-MATURE MONOLAYER AFTER 7 DAYS IN CULTURE

Cardiomyocytes form a uniform, structurally-mature, and highly-organized monolayer after 7 days in culture. Here, phalloidin staining shows the high degree of alignment of cytoskeletal components within cells and within monolayers, relative to cardiomyocytes on Matrigel. Moreover, cardiomyocytes on CELLvo™ Matrix Plus express uniformly high levels of cardiac troponin I (cTnI), relative to Matrigel. This is especially interesting considering that this gene is not expressed prenatally.